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Interleukin-2 production by cultured human granulosa cells
R Orvieto1, A R Genazzani, F Petraglia
1Department of Obstetrics and Gynecology, Rabin Medical Center-Campus Golda (Hasharon Hospital), Petah Tikva, Israel.
Objective:
To determine whether cultured human granulosa cells (GC) produce Interleukin (IL)-2 and whether this GC IL-2 production may be regulated by human chorionic gonadotropin (hCG).
Materials And Methods:
Human GC derived from preovulatory follicles during in vitro fertilization (IVF) cycles were cultured in the absence or presence of hCG. Interleukin-2 was measured in tissue-culture medium by an enzyme-linked immunosorbent assay (ELISA) system.
Results:
Cultured GCs were demonstrated to produce IL-2 in vitro. Moreover, a positive, but not statistically significant, dose-response effect was demonstrated between culture supernatant IL-2 levels and hCG concentration.
Conclusions:
Our data indicate that human GC produce IL-2 and that this production might be regulated by hCG. These results lend credence to the theory that IL-2 might be involved in the events leading to ovarian hyperstimulation syndrome (OHSS).