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Displacement chromatography with on-column isomerization

A S Rathore1, C Horváth

  • 1Department of Chemical Engineering, Yale University, New Haven, CT 06520, USA.

Journal of Chromatography. A
|December 31, 1997
PubMed
Summary
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Simulating displacement chromatography for peptides with reversible reactions, this study found temperature significantly impacts separation yield and production rate. Optimal conditions for yield differ from those for production rate, highlighting key operational variables.

Area of Science:

  • Biochemical Engineering
  • Separation Science
  • Chromatography

Background:

  • Peptide isomerization, particularly cis-trans forms involving peptidyl-proline residues, complicates chromatographic separation.
  • Langmuirian adsorption and reversible first-order reactions are key factors in the separation of interconverting components.

Purpose of the Study:

  • To simulate displacement chromatography for separating two interconverting components with Langmuirian adsorption.
  • To evaluate the impact of operational parameters like temperature, column length, flow velocity, and displacer concentration on separation yield and production rate.

Main Methods:

  • Finite difference scheme was employed for concentration profile computation.
  • Simulations utilized parameter values relevant to reversed-phase chromatography and capillary electrophoresis of phenylalanine-proline dipeptide.

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Main Results:

  • Temperature is the most critical operational variable, influencing both kinetic and adsorption parameters.
  • Yield consistently decreases with increasing temperature (2-42°C).
  • Optimal conditions for yield and production rate were found to be significantly different.

Conclusions:

  • Efficient separation requires minimizing interconversion rates through careful selection of pH, solvent, and temperature.
  • Higher temperatures can be beneficial for optimizing production rate, despite negatively impacting yield.