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Granzyme B immunoreactivity in T/natural killer cell lymphomas
1Department of Pathology, Yonsei University College of Medicine, Seoul, Korea.
Yonsei Medical Journal
|December 31, 1997
Summary
Granzyme B, a protease in cytotoxic lymphocytes, is a valuable diagnostic marker for T/NK-cell lymphomas. Immunohistochemistry using the GrB-7 antibody aids in identifying these lymphomas in routinely processed tissue sections.
Area of Science:
- Immunology
- Oncology
- Pathology
Background:
- Granzyme B is a serine protease found in cytotoxic lymphocytes like NK cells and T-cells.
- Accurate diagnosis of T/NK-cell lymphomas is crucial for effective treatment.
Purpose of the Study:
- To assess the utility of granzyme B immunoreactivity in diagnosing T/NK-cell lymphomas.
- To evaluate the effectiveness of the GrB-7 monoclonal antibody in paraffin-embedded tissues.
Main Methods:
- Paraffin-section immunohistochemistry was performed on 69 upper aerodigestive tract lymphoma cases.
- A monoclonal antibody (GrB-7) targeting granzyme B was used.
- Immunoreactivity was analyzed in conjunction with CD56, CD3, and CD45RO markers.
Main Results:
- All 19 T/NK-cell lymphomas (CD56+, CD3+/CD45RO+) showed granular cytoplasmic granzyme B.
- Two of nine T-cell lymphomas lacking CD56 expression were also granzyme B positive.
- No granzyme B expression was observed in B-cell or null cell lymphomas, apart from reactive cells.
Conclusions:
- Monoclonal antibody GrB-7 is a sensitive and useful marker for diagnosing T/NK-cell lymphomas.
- Granzyme B can be detected in routinely processed tissues without special retrieval techniques.
- Combined use of granzyme B and CD56 enhances diagnostic accuracy for T/NK-cell lymphomas.