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cDNA cloning of the translocon associated protein beta-subunit in the chick cerebellum
I Zarkadis1, E Vezyri, E Kouvelas
1Department of Biology, Medical Faculty, University of Patras, Greece.
Gene
|December 31, 1997
Abstract:
The 'translocon associated protein' is a tetrameric complex residing in the translocation sites, in which nascent polypeptides pass through the endoplasmic reticulum membrane. The beta subunit of this complex is a single spanning membrane protein, as deduced from cDNAs deriving from canine or human epithelial tissues. We have isolated and analysed a cDNA clone of the beta subunit from chick nervous tissue, namely cerebellum. Its deduced protein sequence is 91% homologous to both the canine and the human protein sequences, showing that the molecule is highly conserved.