Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[A cross-bridge model for the artificial mobile systems]

G I Mashanov

    Biofizika
    |December 31, 1997
    PubMed
    Summary
    This summary is machine-generated.

    A new kinetic model for the actin-myosin cross-bridge cycle was developed using caged ATP and limited myosin experiments. This model accurately reflects in vitro motility assay results under external load conditions.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Automatic tracking of individual migrating cells using low-magnification dark-field microscopy.

    Journal of microscopy·2012
    Same author

    Automatic detection of single fluorophores in live cells.

    Biophysical journal·2007
    Same author

    Cell biochemistry studied by single-molecule imaging.

    Biochemical Society transactions·2006
    Same author

    [Visualizing single fluorophores in live cells].

    Biofizika·2006
    Same author

    Visualizing single molecules inside living cells using total internal reflection fluorescence microscopy.

    Methods (San Diego, Calif.)·2003
    Same author

    Muscle force is generated by myosin heads stereospecifically attached to actin.

    Nature·1997
    Same journal

    [On Atomic Nuclear Fusion Processes at Low-Temperatures. An Enhancement of the Probability of Transition through a Potential Barrier Due to the So-Called Barrier Anti-Zeno Effect].

    Biofizika·2016
    Same journal

    [An Iterative Continuous-Event Model of the Population Outbreak of Phytophagous Hemiptera].

    Biofizika·2016
    Same journal

    [Providing the Optimal Insolation of a Photobiological Architectural Shell for Microalgae Cultivation].

    Biofizika·2016
    Same journal

    [Neurodynamic Bases of Imitation Learning and Episodic Memory].

    Biofizika·2016
    Same journal

    [Some Approaches to Activation of Antitumor Resistance Mechanisms and Functional Analogs in Categories of Synergetics].

    Biofizika·2016
    Same journal

    [Mechanism of Formation of Cardiac Arrhythmia Due to Pathological Distribution of Myocardium Conductivity].

    Biofizika·2016
    See all related articles

    Area of Science:

    • Biophysics
    • Biochemistry
    • Molecular Biology

    Context:

    • The actin-myosin interaction is fundamental to muscle contraction and cellular motility.
    • Understanding the mechano-chemical cycle of myosin cross-bridges is crucial for elucidating muscle function.
    • Previous models often simplify the complex dynamics of myosin head interactions.

    Purpose:

    • To propose a simple kinetic scheme for the mechano-chemical cycle of the actin-myosin cross-bridge.
    • To validate the proposed model against experimental data from motility assays using caged ATP and limited myosin molecules.
    • To provide a tool for investigating actin-myosin complexes under external load.

    Summary:

    • A straightforward kinetic model of the cross-bridge mechano-chemical cycle was developed.

    Related Experiment Videos

  • The model was validated using experiments with caged ATP and a limited number of myosin molecules in vitro.
  • The model successfully reproduces experimental results from in vitro motility assays.

    • Impact:

    • This model offers a simplified yet effective framework for understanding myosin motor function.
    • It enables the investigation of actin-myosin complex behavior under varying external load conditions.
    • The findings contribute to a deeper comprehension of muscle mechanics and molecular motor dynamics.