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Machines for automated evolution experiments in vitro based on the serial-transfer concept

G Strunk1, T Ederhof

  • 1Max-Planck-Institute für biophysikalische Chemie, Evolutionsgruppe, Am Fassberg 11, Göttingen, Germany.

Biophysical Chemistry
|June 30, 1997
PubMed
Summary

Automated machines enable real-time monitoring of in vitro evolution experiments for RNA and DNA. These systems facilitate high-throughput screening and the study of molecular adaptation under changing environmental conditions.

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Evolutionary Biology

Background:

  • Automated systems are crucial for advancing in vitro evolution studies.
  • Real-time monitoring and controlled conditions are essential for accurate evolutionary experiments.

Purpose of the Study:

  • To describe two novel machine setups for automated in vitro evolution experiments.
  • To enable real-time monitoring and high-throughput screening of molecular evolution.

Main Methods:

  • Utilized high-sensitivity glass fiber laser fluorimeters and automated microliter sample handling.
  • Implemented a modified serial-transfer technique for constant growth conditions with large populations.
  • Developed single-channel and multichannel machines for monitoring isothermal amplification reactions and PCR.

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Main Results:

  • Demonstrated rapid evolution of an RNA quasi-species under increasing ribonuclease A selection pressure (approx. 80 generations).
  • The multichannel machine allows for high-throughput screening of up to 960 samples simultaneously.
  • Confirmed suitability for uniform sample processing and thermocycling in PCR amplifications.

Conclusions:

  • The described machines significantly enhance the capabilities for conducting automated in vitro evolution experiments.
  • These systems facilitate the investigation of molecular adaptation and high-throughput screening of evolutionary processes.