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Related Experiment Videos

A rat model with an isolated bladder in situ

J Thulesen1, P S Olsen, J U Grevstad

  • 1Department B, University of Copenhagen, Panum Institute, Denmark.

Surgery Today
|January 1, 1997
PubMed
Summary

Researchers developed a novel rat model with an isolated bladder in situ, preventing urine contact. This innovative model aids in studying bladder and urothelium responses to specific compounds, including carcinogens.

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Area of Science:

  • Urology
  • Surgical Innovation
  • Animal Models

Background:

  • Investigating bladder physiology and disease requires relevant experimental models.
  • Current models may not fully replicate the native bladder environment for specific studies.

Purpose of the Study:

  • To describe a novel surgical method for creating an isolated bladder in situ rat model.
  • To provide a tool for studying the effects of various compounds on the bladder urothelium without urine exposure.

Main Methods:

  • Surgical creation of an isolated bladder in situ in rats.
  • Right nephrectomy followed by ureteral catheterization for bladder infusions.
  • Anastomosis of the left ureter to the descending colon to divert urine.
  • Daily saline infusions to maintain bladder viability and prevent atrophy.

Main Results:

  • Successful creation of a rat model with an isolated bladder in situ.
  • The model prevents direct contact between urine and the bladder urothelium.
  • The model allows for controlled infusion of substances into the bladder.

Conclusions:

  • This isolated bladder in situ rat model is a valuable tool for bladder research.
  • It enables the study of specific compound effects on the bladder and urothelium.
  • The model facilitates research into bladder diseases and potential therapeutic interventions.

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