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Small cell lung cancer can express CD34 antigen
1Second Department of Medicine, Okayama University Medical School, Japan.
Anticancer Research
|December 31, 1997
Summary
Small cell lung cancer (SCLC) cells can express CD34, a marker typically found on hematopoietic stem cells. This finding is crucial for autotransplantation, requiring screening for CD34 expression on SCLC tumors.
Area of Science:
- Oncology
- Hematology
Background:
- CD34 is a validated marker for hematopoietic stem and progenitor cells.
- Solid tumors generally lack CD34 expression, enabling its use in purging malignant cells during autotransplantation.
Purpose of the Study:
- To investigate CD34 expression on small cell lung cancer (SCLC) cells.
- To determine the implications of CD34 expression on SCLC for autotransplantation strategies.
Main Methods:
- Analysis of eleven SCLC cell lines using flow cytometry.
- Confirmation of CD34 expression using reverse transcriptase and polymerase chain reaction (RT-PCR).
Main Results:
- Two out of eleven SCLC cell lines exhibited positive CD34 expression.
- Full-length CD34 expression was confirmed in these cell lines via RT-PCR.
Conclusions:
- CD34 expression is present on some SCLC tumor cells, contrary to general assumptions.
- Pre-transplantation screening for CD34 on SCLC cells is essential to ensure effective autotransplantation and avoid contamination with malignant cells.