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Genomic (5'UTR) and serological differences among German BVDV field isolates
1Institute of Medical Microbiology, Infectious and Epidemic Diseases, Ludwig-Maximilians-University Munich, Germany.
Archives of Virology
|January 1, 1997
Summary
Genomic analysis of German bovine viral diarrhoea virus (BVDV) isolates revealed genetic heterogeneity. Polymerase chain reaction (PCR) identified BVDV II in 11 of 96 samples, highlighting distinct viral genotypes.
Area of Science:
- Veterinary Virology
- Molecular Epidemiology
Background:
- Bovine viral diarrhoea virus (BVDV) is a significant pathogen in cattle.
- Understanding BVDV genetic diversity is crucial for effective control strategies.
Purpose of the Study:
- To investigate the genomic heterogeneity of BVDV isolates from Germany.
- To differentiate between BVDV I and BVDV II genotypes using molecular methods.
Main Methods:
- Analysis of the 5' untranslated region (UTR) and NS2-3/4A gene sequences.
- Polymerase chain reaction (PCR) for genotyping.
- Virus neutralization tests using type-specific antisera.
Main Results:
- Eleven out of 96 German BVDV isolates were identified as BVDV II.
- Significant reduction in virus neutralization titers observed with heterologous antisera, confirming genetic distinctness.
Conclusions:
- BVDV II circulates in Germany, indicating the need for genotype-specific diagnostics and vaccines.
- Genomic analysis of the 5'UTR is effective for differentiating BVDV types.
