Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Analysis of neurosteroids]

K Shimada1

  • 1Faculty of Pharmaceutical Sciences, Kanazawa University, Japan.

Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan
|January 1, 1998
PubMed
Summary

Neurosteroids are synthesized within the nervous system and accumulate independently of glands. This review details methods for characterizing these brain steroids, including fatty acid esters.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Selection of methods for diagnosis and treatment of pancreatic cancer].

Gan to kagaku ryoho. Cancer & chemotherapy·1992
Same author

Photoaffinity labeling of the phylloquinone-binding polypeptides by 2-azidoanthraquinone in photosystem I particles.

FEBS letters·1992
Same author

In vitro susceptibility of clinical isolates of Bacteroides fragilis and Bacteroides thetaiotaomicron in Japan.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·1992
Same author

Matlystatins, new inhibitors of typeIV collagenases from Actinomadura atramentaria. II. Biological activities.

The Journal of antibiotics·1992
Same author

[Effectiveness of intraoperative cytological examination of peritoneal washings for patients with pancreatic cancer].

Nihon Geka Gakkai zasshi·1992
Same author

Color Doppler flow imaging of hepatic focal nodular hyperplasia.

Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine·1992

Area of Science:

  • Neuroendocrinology and Steroid Biochemistry
  • Analysis of neurosteroids and their conjugates in the central nervous system.

Context:

  • Neurosteroids are endogenous steroids synthesized within the brain, distinct from peripheral steroid hormones.
  • Their levels in the nervous system can be independent of endocrine gland secretion.

Purpose:

  • To review the characterization and determination methods for neurosteroids and their conjugates in the brain.
  • To highlight analytical techniques like LC/APCI-MS for identifying neurosteroid fatty acid esters.

Summary:

  • Neurosteroids are synthesized de novo or from precursors in the nervous system and accumulate independently of peripheral glands.
  • They exist in various forms including unconjugated steroids, sulfates, fatty acid esters, and sulfolipids.
  • Specific methods, such as LC/APCI-MS, are employed for the separation, derivatization, and identification of neurosteroid conjugates like pregnenolone and dehydroepiandrosterone fatty acid esters in rat brains.

Impact:

  • Provides a comprehensive overview of neurosteroid analysis, crucial for understanding their roles in neurological functions.
  • Establishes analytical frameworks for identifying and quantifying these vital brain compounds.
  • Facilitates further research into the physiological functions and potential therapeutic applications of neurosteroids.

Related Experiment Videos