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Isolating And Immunostaining Lymphocytes and Dendritic Cells from Murine Peyer's Patches
Published on: March 17, 2013
Peyer's patch organogenesis--cytokines rule, OK?
1Department of Microbiology and Immunology, University of Adelaide, South Australia.
Gut
|January 1, 1998
Summary
Tumor necrosis factor (TNF) signaling is not essential for lymphoid organ development but is crucial for organizing B cell follicles. This research clarifies the distinct roles of TNF and lymphotoxin signaling in immune system structure.
Area of Science:
- Immunology
- Molecular Biology
- Developmental Biology
Background:
- The tumor necrosis factor (TNF)/lymphotoxin (LT) system is vital for immune organ development.
- Lymphotoxin-alpha beta (LT alpha beta)/lymphotoxin-beta receptor (LT beta-R) signaling is critical for lymph node and Peyer's patch organogenesis.
- TNF/p55 tumor necrosis factor receptor (p55TNF-R) signaling is implicated in splenic B cell follicle and germinal center formation.
Purpose of the Study:
- To investigate the role of TNF/p55TNF-R signaling in Peyer's patch organogenesis.
- To determine the necessity of TNF/p55TNF-R signaling for lymphocyte segregation and dendritic cell organization in secondary lymphoid tissues.
- To elucidate the specific functions of TNF/p55TNF-R signaling in B cell follicle and follicular dendritic cell network formation.
Main Methods:
- Gene knockout studies using TNF and p55TNF-R deficient mice.
- Analysis of lymphoid tissue development and organization (lymph nodes, Peyer's patches, spleen).
- Assessment of lymphocyte homing, segregation, and dendritic cell localization.
Main Results:
- Peyer's patches develop normally in TNF and p55TNF-R knockout mice, indicating these molecules are not essential for their organogenesis.
- Lymphocyte segregation into T and B cell areas and dendritic cell organization are normal in lymph nodes and Peyer's patches lacking TNF/p55TNF-R signaling.
- Despite normal B cell homing, organized follicular structures and follicular dendritic cell networks fail to form in the absence of TNF/p55TNF-R signaling.
Conclusions:
- TNF/p55TNF-R signaling is not required for the organogenesis of secondary lymphoid tissues like Peyer's patches.
- TNF signaling through p55TNF-R is essential for establishing organized B cell follicles and follicular dendritic cell networks in all secondary lymphoid tissues.
- Distinct roles exist for LT alpha beta and TNF signaling in immune system organization: LT alpha beta for organogenesis and TNF for B cell follicle structure.
