Cardiodepressor and vasodepressor activities of partially purified bovine hypothalamic extract

About 2000 bovine hypothalami were homogenized, extracted, purified and three times filtrated on Sephadex G - 25 column. During the first and second filtration the fractions with vasodepressor activity were collected for further purification. During the third filtration about 40 fractions possessing vasodepressor activity were found. The fractions emerging before vasopressin decreased the arterial blood pressure and induced transient bradycardia in rats. The results indicate that some fractions of hypothalamic extract produce vasodepressor response by vasodilatation only and the other ones by simultaneous vasodilatation and heart rate decrease.