Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

RANTES and MIP-1alpha activate stats in T cells

M Wong1, E N Fish

  • 1Department of Immunology, University of Toronto, Toronto, Ontario M5S 3E2, Canada.

The Journal of Biological Chemistry
|February 7, 1998
PubMed
Summary

Chemokines like RANTES and MIP-1alpha activate T cells by engaging specific receptors. This process rapidly activates transcription factors Stat1 and Stat3, leading to gene expression and T cell function regulation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Human SLFN5 is a transcriptional co-repressor of STAT1-mediated interferon responses and promotes the malignant phenotype in glioblastoma.

Oncogene·2017
Same author

LAPCs contribute to the pathogenesis of allergen-induced allergic airway inflammation in mice.

Allergy·2014
Same author

Beta interferon regulation of glucose metabolism is PI3K/Akt dependent and important for antiviral activity against coxsackievirus B3.

Journal of virology·2014
Same author

SeXX matters in immunity.

Trends in immunology·2013
Same author

Using real-time alerts for clinical trials: Identifying potential study subjects.

Applied clinical informatics·2013
Same author

Interferon-beta is a key regulator of proinflammatory events in experimental autoimmune encephalomyelitis.

Multiple sclerosis (Houndmills, Basingstoke, England)·2010

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Signaling

Background:

  • Chemokines RANTES and MIP-1alpha are key regulators of T cell functions.
  • T cell activation by RANTES involves G protein and protein-tyrosine kinase signaling pathways.

Purpose of the Study:

  • To investigate the role of transcription factors Stat1 and Stat3 in T cell activation by RANTES and MIP-1alpha.
  • To elucidate the signaling pathways linking chemokine receptors to Stat activation and gene expression.

Main Methods:

  • Treatment of T cell lines (MOLT-4, Jurkat) with RANTES and MIP-1alpha.
  • Analysis of Stat1 and Stat3 activation using Western blotting and DNA-binding assays.
  • Assessment of c-fos gene induction via transcriptional activation.
  • Investigation of chemokine receptor (CCR1, CCR4, CCR5) expression.

Main Results:

  • RANTES and MIP-1alpha rapidly activate Stat1 and Stat3 transcription factors in T cells.
  • Tyrosine-phosphorylated Stat1 dimers bind DNA following chemokine stimulation.
  • Chemokine treatment induces transcriptional activation of the Stat-inducible gene c-fos.
  • Stat activation correlates with c-fos induction and expression of CCR4 and CCR5.

Conclusions:

  • Chemokine signaling through CCR1, CCR4, and CCR5 activates Stat1 and Stat3 in T cells.
  • This activation leads to downstream gene expression, such as c-fos, influencing T cell function.
  • Chemokine receptors utilize signaling intermediates common to other cytokine receptors.

Related Experiment Videos