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Streptomyces coelicolor DNA homologous with acyltransferase domains of type I polyketide synthase gene complex
K Kuczek1, K Pawlik, M Kotowska
1Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland. kuczek@immuno.iitd.pan.wroc.pl
Abstract:
An acyltransferase-homologous DNA fragment was amplified in a PCR reaction on a cosmid DNA template from the genomic DNA library of the soil bacterium Streptomyces coelicolor A3(2). The putative amino acid sequence of the fragment resembles acyl-CoA:ACP acyltransferase domains from several bacterial enzymatic complexes of polyketide synthase. There is a high similarity with acyltransferase domains from so-called type I polyketide synthases. Such synthases catalyze production of the aglycone portion of macrolides and polyethers that are important as antibiotics or immunosuppressants. The amplified fragment is considered to be a part of a larger gene complex.
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