Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Cations in the rat pars distalis ultrastructural localization

J E Schechter

    The American Journal of Anatomy
    |June 1, 1976
    PubMed
    Summary

    Calcium ions are crucial for pituitary gland secretory cells, facilitating the transport and release of granules. This study visualizes calcium localization within rat pituitary cells using electron microscopy, revealing its role in exocytosis.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Systematic variations in immune response-related gene transcript abundance suggest new questions about environmental influences on lacrimal gland immunoregulation.

    Current eye research·2011
    Same author

    Suppression of lymphocyte proliferation and regulation of dendritic cell phenotype by soluble mediators from rat lacrimal epithelial cells.

    Scandinavian journal of immunology·2009
    Same author

    Autoimmune dacryoadenitis and keratoconjunctivitis induced in rabbits by subcutaneous injection of autologous lymphocytes activated ex vivo against lacrimal antigens.

    Journal of autoimmunity·2008
    Same author

    Biochemical changes contributing to functional quiescence in lacrimal gland acinar cells after chronic ex vivo exposure to a muscarinic agonist.

    Scandinavian journal of immunology·2003
    Same author

    Lacrimal gland epithelial cells stimulate proliferation in autologous lymphocyte preparations.

    Experimental eye research·2000
    Same author

    Autologous lacrimal-lymphoid mixed-cell reactions induce dacryoadenitis in rabbits.

    Experimental eye research·2000

    Area of Science:

    • Cell Biology
    • Endocrinology
    • Neuroscience

    Background:

    • The pituitary gland plays a vital role in hormone regulation.
    • Understanding the intracellular mechanisms of secretory granule transport and release is essential.

    Purpose of the Study:

    • To investigate the localization and role of cations, particularly calcium, within the secretory cells of the rat pituitary gland.

    Main Methods:

    • Utilized the potassium pyroantimonate-osmium tetroxide fixation technique for electron microscopy.
    • Examined cation localization in the pars distalis of female Sprague Dawley rats.
    • Employed EGTA and EDTA chelating agents to identify specific cations.

    Main Results:

    • Electron-dense precipitate, indicative of cation localization, was observed in nuclei, endoplasmic reticulum, mitochondria, and Golgi apparatus.
    • Precipitate was found within secretory granules at various maturation stages and near the plasma membrane.
    • Evidence of vesicle fusion with granules and precipitate clustering during emiocytosis (granule release) was noted.
    • Calcium was identified as the primary cation in the cytoplasm, associated with secretory processes.

    Conclusions:

    • Calcium ions are significantly localized within pituitary secretory cells.
    • Calcium likely facilitates the transport and release of secretory granules via emiocytosis.
    • This study provides ultrastructural evidence for calcium's role in pituitary hormone secretion.

    Related Experiment Videos