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Pregnancy after immature oocyte donation and intracytoplasmic sperm injection
1Department of Obstetrics and Gynecology, Shin Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan, Republic of China.
Objective:
To report a case of pregnancy from in vitro-matured primary oocytes fertilized by ICSI. The pregnancy occurred in a woman who was in an oocyte donation program; the woman's husband had normal sperm parameters.
Design:
Case report.
Setting:
Private general hospital affiliated with a university hospital.
Patient(S):
A recipient with premature ovarian failure, a recipient's husband with normal sperm, and a pregnant woman who donated her oocytes.
Intervention(S):
Aspiration of immature oocytes during cesarean section, in vitro culture for maturation, ICSI of matured oocytes, coculture of fertilized oocytes.
Main Outcome Measure(S):
Fertilization of oocytes by ICSI, and cleavage of embryos by Vero cell coculture.
Result(S):
Two of seven immature oocytes became metaphase II oocytes, and both were fertilized by ICSI. The two zygotes were cocultured on Vero cells to become grade 1 two-cell embryos. Pregnancy was obtained after transfer.
Conclusion(S):
More studies are necessary to clarify whether ICSI can increase the fertilization rate of in vitro-matured primary oocytes, and to clarify the role of coculture in fertilization.