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Steroid receptors in surgical pathology. With special reference to small mammographically detected breast lesions
J C Ross1, S Binder, M C Rounsaville
1Department of Anatomic Pathology, California Pacific Medical Center, San Francisco 94118, USA.
Abstract:
The cellular receptors for estrogen, progesterone, and other steroid hormones appear in the cytosol fraction of homogenated specimens of hormonal target tissues. These receptors have traditionally been detected and quantitated by biochemical techniques that involve binding tritium-labelled marker steroids to the receptor proteins. An important recent development has been the creation of various monoclonal antibody reagents that specifically recognize antigenic determinants on receptor molecules.
Insights
Cellular receptors for steroid hormones like estrogen and progesterone are found in target tissues. New monoclonal antibodies offer a precise method for detecting and quantifying these crucial hormone receptors.
Area of Science:
- Endocrinology
- Molecular Biology
- Cell Biology
Background:
- Cellular receptors for steroid hormones (estrogen, progesterone) are located in the cytosol of target tissues.
- Traditional detection methods rely on biochemical assays using tritium-labeled steroids.
- These methods quantify receptor proteins by measuring their binding affinity.
Purpose of the Study:
- To introduce and highlight the significance of monoclonal antibodies for steroid hormone receptor analysis.
- To present an alternative to traditional biochemical detection methods.
Main Methods:
- Utilizing monoclonal antibody reagents that specifically target antigenic determinants on receptor molecules.
- Developing assays based on antibody recognition of receptor proteins.
Main Results:
- Monoclonal antibodies provide specific recognition of steroid hormone receptor molecules.
- This enables precise detection and quantification of receptors.
Conclusions:
- Monoclonal antibodies represent a significant advancement in steroid hormone receptor research.
- These reagents offer improved specificity and accuracy for receptor detection and quantification.