Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Stem cells: characterization and measurement

R E Ploemacher1

  • 1Department of Haematology, Erasmus University, Rotterdam, The Netherlands.

Bailliere'S Clinical Haematology
|January 9, 1998
PubMed
Summary

Haemopoietic stem cells (HSCs) sustain blood formation throughout life. Their function and regulation by the stroma are key for gene therapy and transplantation, though assays need further validation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Toward a myeloablative regimen with clinical potential: II. Treosulfan induces specific skin graft tolerance across haploidentical MHC barriers.

Bone marrow transplantation·2003
Same author

Towards a myeloablative regimen with clinical potential: I. Treosulfan conditioning and bone marrow transplantation allow induction of donor-specific tolerance for skin grafts across full MHC barriers.

Bone marrow transplantation·2003
Same author

Primary murine MSC show highly efficient homing to the bone marrow but lose homing ability following culture.

Leukemia·2003
Same author

Stromal cells from murine embryonic aorta-gonad-mesonephros region, liver and gut mesentery expand human umbilical cord blood-derived CAFC(week6) in extended long-term cultures.

Leukemia·2002
Same author

Stromal support augments extended long-term ex vivo expansion of hemopoietic progenitor cells.

Leukemia·2001
Same author

Improved prognostic significance of cytokine-induced proliferation in vitro in patients with de novo acute myeloid leukemia of intermediate risk: impact of internal tandem duplications in the Flt3 gene.

Leukemia·2001

Area of Science:

  • Hematology
  • Stem Cell Biology
  • Gene Therapy

Background:

  • Blood formation relies on a small population of haemopoietic stem cells (HSCs).
  • HSCs exhibit heterogeneity in proliferative capacity, impacting their contribution to blood chimerism.
  • The precise phenotype and reliable quantification of HSCs remain challenging.

Purpose of the Study:

  • To explore the regulatory mechanisms of HSC quiescence and proliferation.
  • To investigate the role of the stroma and its derived factors in HSC regulation.
  • To assess the potential of HSCs in gene therapy and transplantation.

Main Methods:

  • Phenotypic analysis (morphological, flow cytometry) of HSCs.
  • Functional HSC assays: in vitro (e.g., CAFC, LTC-IC) and in vivo (e.g., NOD/SCID mouse repopulation).
  • Investigation of stroma-HSC interactions and the influence of stroma-derived factors.

Main Results:

  • Functional assays may offer superior assessment of HSCs compared to phenotypic analysis, though validation in human transplants is pending.
  • HSC quiescence and proliferation are significantly regulated by the haemopoietic organ stroma.
  • Stroma-derived factors likely play crucial roles in HSC regulation, with evidence suggesting enhanced progeny production in direct stroma contact.

Conclusions:

  • HSCs are critical for lifelong blood formation and possess self-renewal capabilities, making them attractive for gene therapy.
  • Further characterization of stroma-derived factors is needed to fully understand HSC regulation.
  • Improved understanding and manipulation of HSCs hold promise for enhanced hematopoietic recovery and transplantation strategies.

Related Experiment Videos