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Amperometric glutamate biosensor based on poly(o-phenylenediamine) film electrogenerated onto modified carbon paste
S L Alvarez-Crespo1, M J Lobo-Castañón, A J Miranda-Ordieres
1Departamento de Química Física y Analítica, Universidad de Oviedo, Asturias, Spain.
Abstract:
A glutamate biosensor was developed by electropolymerizing o-phenylenediamine on a dehydrogenase-NAD+ modified carbon paste electrode. The amperometric response to glutamate was obtained by means of the electrocatalytic oxidation of the enzymatically produced NADH, at an applied potential close to 0 V (Ag/AgCl). After optimizing carbon paste composition, polymer thickness and operating variables, a linear amperometric response to glutamate was obtained within the concentration range 5.0 x 10(-6)-7.8 x 10(-5) M with a detection limit of 3.8 x 10(-6) M. The biosensor was applied to the determination of glutamate in chicken bouillon cubes. Good accuracy was found with respect to a reference enzymatic spectrophotometric method.