Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Human Saphenous Vein Endothelial Cell Isolation and Exposure to Controlled Levels of Shear Stress and Stretch09:10

Human Saphenous Vein Endothelial Cell Isolation and Exposure to Controlled Levels of Shear Stress and Stretch

1.4K
We describe a protocol to isolate and culture human saphenous vein endothelial cells (hSVECs). We also provide detailed methods to produce shear stress and stretch to study mechanical stress in...
1.4K
Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast11:04

Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast

7.6K
Protein aggregation elicits cellular oxidative stress. This protocol describes a method for monitoring the intracellular states of amyloidogenic proteins and the oxidative stress associated with them, using flow cytometry. The approach is used to study the behavior of soluble and aggregation-prone variants of the amyloid-β...
7.6K
En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries08:58

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries

10.0K
In this article we describe an adapted relatively easy method using the fluorescence dye diaminofluorescein-2 diacetate (DAF-2DA) and dihydroethidium (DHE) for en face simultaneous detection and visualization of intracellular nitric oxide (NO) and superoxide anion (O2.−) respectively, in freshly isolated intact aortas of an obesity mouse...
10.0K
Comet Assay as an Indirect Measure of Systemic Oxidative Stress08:23

Comet Assay as an Indirect Measure of Systemic Oxidative Stress

29.9K
Comet assay measures DNA breaks, induced by different factors. If all factors (except oxidative stress) causing DNA damage are kept constant, the amount of DNA damage is a good indirect parameter of oxidative stress. The goal of this protocol is to use comet assay for indirect measurement of oxidative...
29.9K
Measuring Oxidative Stress Resistance of Caenorhabditis elegans in 96-well Microtiter Plates08:10

Measuring Oxidative Stress Resistance of Caenorhabditis elegans in 96-well Microtiter Plates

16.2K
C. elegans is an attractive model organism to study signal transduction pathways involved in oxidative stress resistance. Here we provide a protocol to measure oxidative stress resistance of C. elegans animals in liquid phase, using several oxidizing agents in 96 well plates.
16.2K
Analysis of Oxidative Stress in Zebrafish Embryos11:05

Analysis of Oxidative Stress in Zebrafish Embryos

38.1K
Here we report a protocol to measure oxidative stress in living zebrafish embryos. This procedure allows reactive oxygen species (ROS) detection in both whole embryo tissues and single-cell populations. This protocol will accomplish both qualitative and quantitative analyses.
38.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tail-Cuff Versus Radiotelemetry to Measure Blood Pressure in Mice and Rats.

Hypertension (Dallas, Tex. : 1979)·2023
Same author

Hope for resistant hypertension through BrigHTN and PRECISION.

Nature reviews. Nephrology·2023
Same author

Differential cell-matrix mechanoadaptations and inflammation drive regional propensities to aortic fibrosis, aneurysm or dissection in hypertension.

Journal of the Royal Society, Interface·2017
Same author

Macrophages come to mind as keys to cognitive decline.

The Journal of clinical investigation·2016
Same author

A common functional promoter variant links CNR1 gene expression to HDL cholesterol level.

Nature communications·2013
Same author

Endothelial mechanotransduction, nitric oxide and vascular inflammation.

Journal of internal medicine·2006
Same journal

In-Hospital Cardiac Arrest Detection Performance Analysis and Comparison on Effective Feature Selection.

Clinical cardiology·2026
Same journal

Interpreting the Association Between Diuretic Intensity Score and Mortality: The Potential Roles of Diuretic Responsiveness and Mineralocorticoid Receptor Antagonist Therapy.

Clinical cardiology·2026
Same journal

Heart Failure With Preserved Ejection Fraction-Like Phenotype in Coronary Artery Disease and Obstructive Sleep Apnea: Insights From the RICCADSA Cohort.

Clinical cardiology·2026
Same journal

Ethanol Infusion Into the Vein of Marshall for Atrial Fibrillation: Clinical Efficacy and Technical Limitations.

Clinical cardiology·2026
Same journal

Methodological Considerations Regarding Diuretic Intensity Score and Mortality in Hospitalized Heart Failure Patients.

Clinical cardiology·2026
Same journal

Inpatient Outcomes and Complications After Left Atrial Appendage Occlusion in Rural Versus Urban Hospitals in the United States.

Clinical cardiology·2026
See all related articles

Related Experiment Video

Updated: Jan 20, 2026

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries
08:58

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries

Published on: February 25, 2016

10.0K

Endothelial function and oxidant stress

D G Harrison1

  • 1Department of Medicine, Emory University School of Medicine, Atlanta, Georgia 30329, USA.

Clinical Cardiology
|January 10, 1998
PubMed
Summary
This summary is machine-generated.

Vascular cells produce reactive oxygen species, impacting nitric oxide levels and vasomotion, particularly in diseases like atherosclerosis and hypertension. A key enzyme, NADH oxidase, is implicated in this process.

More Related Videos

Comet Assay as an Indirect Measure of Systemic Oxidative Stress
08:23

Comet Assay as an Indirect Measure of Systemic Oxidative Stress

Published on: May 22, 2015

29.9K
Analysis of Oxidative Stress in Zebrafish Embryos
11:05

Analysis of Oxidative Stress in Zebrafish Embryos

Published on: July 7, 2014

38.1K

Related Experiment Videos

Last Updated: Jan 20, 2026

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries
08:58

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries

Published on: February 25, 2016

10.0K
Comet Assay as an Indirect Measure of Systemic Oxidative Stress
08:23

Comet Assay as an Indirect Measure of Systemic Oxidative Stress

Published on: May 22, 2015

29.9K
Analysis of Oxidative Stress in Zebrafish Embryos
11:05

Analysis of Oxidative Stress in Zebrafish Embryos

Published on: July 7, 2014

38.1K

Area of Science:

  • Cardiovascular Biology
  • Oxidative Stress Research
  • Vascular Physiology

Background:

  • Endothelial cells and vascular smooth muscle cells generate reactive oxygen species (ROS) from various enzymatic sources.
  • In conditions like atherosclerosis and hypertension, vascular ROS production significantly increases.
  • Elevated superoxide anion levels deplete nitric oxide, impairing endothelial regulation of vasomotion.

Purpose of the Study:

  • To investigate the primary enzymatic source of vascular superoxide and hydrogen peroxide.
  • To understand the regulatory mechanisms of this enzyme system, particularly by angiotensin II.
  • To explore the link between altered vascular oxidant states and pathologies such as hypertension and atherosclerosis.

Main Methods:

  • Focus on identifying the major enzymatic source of vascular ROS.
  • Investigating the role of membrane-bound, reduced nicotinamide-adenine dinucleotide (NADH)-dependent oxidase.
  • Examining the influence of angiotensin II and nitroglycerin on enzyme activity.

Main Results:

  • Recent evidence points to a membrane-bound, NADH-dependent oxidase as the major source of vascular superoxide and hydrogen peroxide.
  • The activity of this oxidase is regulated by angiotensin II.
  • Enzyme activity is elevated after prolonged nitroglycerin exposure.

Conclusions:

  • The identified NADH oxidase is a critical source of vascular ROS, influencing nitric oxide bioavailability.
  • Angiotensin II regulation of this oxidase suggests a significant role in vascular pathology.
  • This mechanism may link hypertension and atherosclerosis through altered vascular oxidant states.