Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Surrogate antigen processing mediated by TAP-dependent antigenic peptide secretion

R Gabathuler1, J Alimonti, Q J Zhang

  • 1Biotechnology Laboratory, University of British Columbia, Vancouver, British Columbia, Canada V6T1Z3.

The Journal of Cell Biology
|February 14, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characteristics of photosynthetic function in different leaf ages of 'Tieton' sweet cherry in a greenhouse.

Photosynthetica·2026
Same author

[Clinical characteristics and genotype analysis of two rare subtypes of acute myeloid leukemia].

Zhonghua yi xue za zhi·2025
Same author

[Application and development of super minimally invasive surgery concept in acoustic neuroma resection].

Zhonghua yi xue za zhi·2024
Same author

[Acute lymphoblastic leukemia with inv(11)(q21q23.3)/KMT2A::MAML2 fusion gene progressed to acute myeloid leukemia: a case report].

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi·2024
Same author

[Study of the effects of dietary patterns on glycemic control in community type 2 diabetic mellitus patients].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2024
Same author

[Long-term evaluation and physical and mental effects of residual tinnitus following treatment of sudden hearing loss].

Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery·2023

Researchers discovered a new pathway for secreting viral peptides using transporters associated with antigen processing (TAP). This novel peptide secretion mechanism is independent of MHC class I molecules and may play a role in immune responses.

Area of Science:

  • Immunology
  • Cell Biology
  • Virology

Background:

  • MHC class I molecules present peptides derived from cytoplasmic proteins on the cell surface.
  • Peptide loading onto MHC class I occurs in the ER via transporters associated with antigen processing (TAP).

Purpose of the Study:

  • To investigate a novel pathway for peptide secretion.
  • To determine the role of TAP transporters and MHC class I in this secretion pathway.

Main Methods:

  • Utilized a new functional assay to track VSV-N derived peptides.
  • Investigated the dependence on TAP transporters and MHC genotype.
  • Assessed the impact of protein secretion inhibitors (BFA), temperature, metabolic poisons (azide), and protein synthesis inhibitors (emetine).

Related Experiment Videos

Main Results:

  • Identified active secretion of VSV-N derived peptides from cells.
  • Demonstrated TAP transporter dependence and MHC genotype independence.
  • Confirmed that MHC class I expression and transport are not required for this secretion.
  • Showed sensitivity to BFA, low temperature, azide, and emetine, indicating a protein secretion pathway.

Conclusions:

  • Established the existence of a novel peptide secretion pathway utilizing TAP transporters.
  • This pathway is distinct from the ER translocon.
  • Proposed 'surrogate antigen processing' as a potential immune mechanism contributing to intercellular peptide signaling.