Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Calmodulin-dependent protein kinase IV during T-cell development

J Krebs1, A Wilson, P Kisielow

  • 1Institute of Biochemistry, Swiss Federal Institute of Technology (ETH), Zurich, Switzerland.

Biochemical and Biophysical Research Communications
|January 13, 1998
PubMed
Summary

Thyroid hormone T3 induces calmodulin-dependent protein-kinase IV (CaMKIV) in fetal rat brain. In fetal thymus, CaMKIV expression is regulated during development and can be induced by fetal calf serum in culture.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The 1999 annual report of the Regional Infant and Child Mortality Review Committee.

South Dakota journal of medicine·2000
Same author

Using content analysis of video-recorded consultations to identify smokers' "readiness" and "resistance" towards stopping smoking.

Patient education and counseling·2000
Same author

Analysis of the effects of treatment of human Schistosoma mansoni infection on the immune response of patients from endemic areas.

Acta tropica·2000
Same author

A comparison of individual and population smoking data from a postal survey and general practice records.

The British journal of general practice : the journal of the Royal College of General Practitioners·2000
Same author

Frequent loss of BRCA1 mRNA and protein expression in sporadic ovarian cancers.

International journal of cancer·2000
Same author

Detection of the exotic mosquito Culex gelidus in the Northern Territory.

Communicable diseases intelligence·2000

Area of Science:

  • Developmental biology
  • Molecular endocrinology
  • Immunology

Background:

  • Calmodulin-dependent protein-kinase IV (CaMKIV) is crucial for calcium-dependent gene expression.
  • Previous studies showed thyroid hormone T3 induces CaMKIV in fetal rat brain.
  • The developmental regulation of CaMKIV in the thymus was previously unknown.

Purpose of the Study:

  • To investigate the expression and regulation of CaMKIV during fetal thymus development.
  • To determine the role of fetal calf serum in CaMKIV induction in mouse fetal thymus organ culture (FTOC).
  • To examine CaMKIV regulation during T-cell development in adult murine thymus.

Main Methods:

  • Primary cell culture of fetal rat brain.
  • Mouse fetal thymus organ culture (FTOC) with varying culture media.

Related Experiment Videos

  • Analysis of CaMKIV expression during different gestational ages and T-cell development stages.

    • Main Results:

    • CaMKIV was not detected in fetal thymus before day 16 of gestation and was upregulated by day 18.
    • CaMKIV was undetectable in FTOC with minimal medium but specifically induced upon addition of fetal calf serum.
    • CaMKIV expression was tightly regulated during T-cell development in adult murine thymus.

    Conclusions:

    • CaMKIV expression is developmentally regulated in the fetal thymus.
    • Fetal calf serum is a key factor for CaMKIV induction in FTOC.
    • CaMKIV regulation is a stringent process during thymic development and T-cell maturation.