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Updated: Aug 14, 2026

Visualization of UV-induced Replication Intermediates in E. coli using Two-dimensional Agarose-gel Analysis
Published on: December 22, 2010
The consequence of translesional replication of unique UV-induced photoproducts
A Gentil1, F Le Page, A Sarasin
1Laboratory of Molecular Genetics, Institut de Recherches sur le Cancer - IFC1 - C.N.R.S., Villejuif, France.
Abstract:
The consequence of translesional replication of unique UV-induced photoproducts is reviewed here. Mutagenesis induced by unique UV-induced lesions, the thymine-thymine dimer (TT) and the thymine-thymine pyrimidine pyrimidone (6-4), [T(6-4)T] carried on single-stranded DNA vectors and replicated in bacteria, yeast and mammalian cells have been considered. It has been found that in all of the three species the (TT) dimers induce a low mutation frequency compared to the (6-4) photoproduct. The molecular analysis of the mutations induced is reported, showing specific differences depending on the species considered.
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