Related Experiment Video
Updated: Jul 22, 2026

Protein-protein Interactions Visualized by Bimolecular Fluorescence Complementation in Tobacco Protoplasts and Leaves
Published on: March 9, 2014
Three kinds of binding site for tentoxin on isolated chloroplast coupling factor 1
1Department of Biology, School of Education, Waseda University, Tokyo, Japan. 695L5192@mn.waseda.ac.jp
Abstract:
Tentoxin binding on chloroplast coupling factor 1 (CF1) was studied using a centrifugation column method followed by HPLC analysis. From non-linear regression analysis of the results, the presence of three types of binding site with the following Kd values was deduced: 6.9x10(-8) M (first site), 1.4x10(-5) M (second site), and 6.3x10(-3) M (third site). The binding of one tentoxin inhibits, that of two tentoxins moderately restores, and that of three tentoxins greatly stimulates the ATPase activity of CF1. The forward rate constant of the binding of tentoxin on the first site was 6.3x10(3) M-1 s-1.
Related Concept Videos
Ligand Binding and Linkage
Protein Transport to the Stroma
Protein complexes called the translocon of the outer chloroplast membrane or TOC complex, and the translocon of the inner chloroplast membrane or TIC complex mediate the...
Protein Transport to the Outer Chloroplast Membrane
Two models describe the mechanism of precursor recognition and entry across the outer membrane through the TOC complex. Model 1 suggests the newly synthesized precursor binds to the TOC receptor 159 and forms a complex.
Allosteric Proteins-ATCase
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis pathway,...
Electron Transport Chain: Complex III and IV
Protein Transport to the Inner Chloroplast Membrane

