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Updated: Jul 23, 2026

Xenopus laevis as a Model to Identify Translation Impairment
Published on: September 27, 2015
mRNA encoding the catalytic subunit of DNA-dependent protein kinase is widely expressed in Xenopus cells
1The Scripps Research Institute, Department of Molecular and Experimental Medicine, La Jolla, CA 92037, USA. plabhart@scripps.edu
Abstract:
Here, the sequence of a Xenopus laevis cDNA encoding the 640 carboxy-terminal amino acids of the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs) is reported. The predicted Xenopus protein segment is 65% identical to the human counterpart. Northern blot analysis indicates that Xenopus DNA-PKcs is encoded by an approx. 13000 nt transcript. DNA-PKcs mRNA is widely expressed in adult tissues as well as in oocytes and embryos. It is also shown that outside the conserved kinase domain, Xenopus DNA-PKcs bears significant similarities to hypothetical 420.8 and 433.2 kDa proteins in yeast species.
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