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Rainbow trout SOX9: cDNA cloning, gene structure and expression

N Takamatsu1, H Kanda, M Ito

  • 1Department of Biosciences, School of Science, Kitasato University, Sagamihara, Kanagawa, Japan. takamatu@jet.sci.kitasato-u.ac.jp

Gene
|January 14, 1998
PubMed
Summary

Researchers isolated rainbow trout SOX9, a gene crucial for development. This SOX9 gene shows significant similarity to human SOX9 and is notably present in the fish

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Area of Science:

  • Molecular biology
  • Genetics
  • Comparative genomics

Background:

  • SOX9 is a transcription factor vital for skeletal and sexual development in vertebrates.
  • Understanding SOX9's role in non-mammalian species provides insights into its evolutionary conservation and functional importance.

Purpose of the Study:

  • To isolate and characterize the rainbow trout SOX9 gene and its encoded protein.
  • To compare the rainbow trout SOX9 gene structure and protein sequence with its human ortholog.

Main Methods:

  • Isolation and characterization of rainbow trout SOX9 cDNA and genomic clones.
  • Amino acid sequence analysis and comparison with human SOX9.
  • Gene structure analysis, focusing on intron-exon boundaries.

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Main Results:

  • Successfully isolated and characterized rainbow trout SOX9 cDNA, encoding a 488 amino acid protein with a High Mobility Group (HMG) box.
  • Rainbow trout SOX9 exhibits 70% amino acid sequence identity to human SOX9.
  • The gene structure of rainbow trout SOX9 is conserved with the human gene, including conserved intron positions.
  • SOX9 mRNA expression was found to be prominent in rainbow trout testis and brain.

Conclusions:

  • The rainbow trout SOX9 gene is structurally and functionally conserved with its human counterpart.
  • The presence of SOX9 in testis and brain suggests potential roles in sexual development and neurological functions in fish.