Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Complement classical pathway expression by human skeletal myoblasts in vitro

J Legoedec1, P Gasque, J F Jeanne

  • 1Institut Fédératif de Recherches Multidisciplinaires sur les Peptides (nø. 23), INSERM U-78, BP 73, Bois-Guillaume, France.

Molecular Immunology
|July 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of Tranexamic Acid for the Reduction of Blood Loss During Burn Surgery in Adults (Tranburn Study).

Annals of burns and fire disasters·2026
Same author

WORK AND QUALITY OF LIFE AFTER BURNS: SMALL BURNS, BIG CONSEQUENCES.

Annals of burns and fire disasters·2024
Same author

Line-field confocal optical coherence tomography for actinic keratosis and squamous cell carcinoma: a descriptive study.

Clinical and experimental dermatology·2021
Same author

Patients with atherosclerotic peripheral arterial disease have a high risk of lung cancer: Systematic review and meta-analysis of literature.

Journal de medecine vasculaire·2021
Same author

Line-field confocal optical coherence tomography of basal cell carcinoma: a descriptive study.

Journal of the European Academy of Dermatology and Venereology : JEADV·2021
Same author

In vivo characterization of healthy human skin with a novel, non-invasive imaging technique: line-field confocal optical coherence tomography.

Journal of the European Academy of Dermatology and Venereology : JEADV·2020
Same journal

m6A modification of LINC00458 enhances HMOX1 stability via ELAVL1 recruitment to promote ferroptosis and aggravate asthma.

Molecular immunology·2026
Same journal

Overexpression of Hes1 inhibits cigarette smoke-induced mitochondrial apoptosis in AT2 cells by activating the Pgc-1α/Tfam signaling pathway.

Molecular immunology·2026
Same journal

Progesterone promotes favorable pregnancy outcomes in recurrent spontaneous, abortion by attenuating NK Cell overactivation and upregulating the cAMP/PKA/CREB signaling axis.

Molecular immunology·2026
Same journal

Oleanolic acid alleviates hepatic fibrosis by inhibiting liver macrophage recruitment and polarization.

Molecular immunology·2026
Same journal

Cordycepin attenuates diabetic nephropathy by dual-pathway activation of TFEB to restore autophagy and ameliorate podocyte injury.

Molecular immunology·2026
Same journal

Endothelial-derived TWEAK drives granulosa cell apoptosis in PCOS via the Fn14-oxidative stress axis.

Molecular immunology·2026
See all related articles

Human myoblasts produce classical complement pathway components, acting as a local source. This suggests complement may play a role in skeletal muscle inflammation and disease.

Area of Science:

  • Immunology
  • Cell Biology
  • Skeletal Muscle Physiology

Background:

  • Human myoblasts exhibit immunological functions in vitro.
  • Previous work showed myoblasts produce alternative complement pathway components.
  • Myoblasts activate the classical pathway but are protected from complement attack by regulators.

Purpose of the Study:

  • To investigate the biosynthesis of classical complement pathway components by skeletal muscle cells.
  • To understand the relationship between myoblasts and the complement system.

Main Methods:

  • In vitro culture of human myoblasts.
  • Analysis of complement component production (C1q, C1r, C1s, C2, C4).
  • Stimulation with interferon-gamma (IFN-γ) to assess synthesis regulation.

Related Experiment Videos

Main Results:

  • Human myoblasts constitutively produced complement components C1q, C1r, C1s, C2, and C4.
  • All synthesized components showed upregulated production after IFN-γ stimulation.

Conclusions:

  • Human myoblasts can serve as a local source of complement components.
  • Complement may be involved in inflammatory and physiopathological processes within skeletal muscle.