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Updated: Aug 19, 2026
Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells
Published on: November 28, 2015
Flow cytometric DNA analysis of pediatric intracranial ependymomas
P K Kotylo1, P B Robertson, N S Fineberg
1Department of Pathology, Indiana University School of Medicine, Indianapolis 46202-5200, USA.
Objective:
To examine the clinicopathologic features and perform flow cytometric DNA analysis of pediatric intracranial ependymomas to determine whether any of these parameters were predictors of clinical outcome.
Methods:
Flow cytometric DNA analysis was performed on 17 paraffin-embedded tumors from patients aged 7 months to 16 years.
Results:
Seven cases were aneuploid, while the remaining 10 were diploid. Proliferative fractions varied from 1% to 17%.
Conclusions:
No correlation between histologic features such as mitotic activity, cellularity, pleomorphism, vascular proliferation, and length of survival was observed. However, the presence of a diploid DNA stemline, elevated proliferative fraction, or young age were associated with a poor clinical outcome and shortened survival times (P < 0.05). Additional studies of larger patient groups with extended follow-up are necessary to confirm these findings.
