Enzyme activities associated with arabitol and mannitol biosynthesis and catabolism in Schizophyllum commune

Enzymes of polyol metabolism were studied in basidiospore germination of Schizophyllum commune during periods of in vivo arabitol and mannitol pool depletion (growth on glucose-asparagine) and during their subsequent synthesis (growth on acetate-NH+4). Optimal conditions for assays were established and specific activities of enzymes employing D-arabitol, D-mannitol, D-ribulose, D-fructose and D-xylulose as substrates. were traced. Inquiries into the products formed during these reactions showed that D-ribulose generated arabitol while D-fructose produced mannitol with D-xylulose giving rise to xylitol. The dehydrogenase reactions were further investigated using polyacrylamide disc gel electrophoresis. Here was revealed the existence of at least two separate enzymatic activities pertaining to the catabolism of arabitol and mannitol. Also noted were the electrophoretic patterns when D-sorbitol, ribitol, xylitol and ethanol were used as substrates