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Inhibition of PCR by agar from bacteriological transport media
1Provincial Laboratory for Public Health of Southern Alberta, Department of Microbiology and Infectious Diseases, University of Calgary, Canada. gibb@acs.ucalgary.ca
Journal of Clinical Microbiology
|February 12, 1998
Abstract:
We observed inhibition of PCR in throat swabs submitted in routine bacteriological transport media. Experimental studies showed that agar, which was extracted with DNA by DNAzol (Gibco BRL, Gaithersburg, Md.), was the inhibitory agent. No inhibitory effect was observed with a transport medium which did not contain agar.

