[The effect of keeping condition and genotype on the electrophoretic properties of pork proteins]
Related Experiment Video
Updated: Oct 30, 2026

Exploring the Longissimus Muscle: Unraveling its Correlation with Meat Quality in Bos indicus and Crossbred Bulls
Published on: July 12, 2024
E Szerdahelyi1, G Hajós, E Dworschák
1Zentralforschungsinstitut für die Lebensmittelindustrie, Budapest.
Abstract:
Besides differentiation of animal species the electrophoretic methods are efficient also in determinations of the factors effecting the protein patterns. The goal of this study is to compare the effect of the genotype and the keeping conditions on the electrophoretic behaviour of the pork meat proteins. Isoelectric focusing was found to be more efficient than SDS-PAGE concerning studies of the effect of these factors. An optimal separation has been achieved with an ampholyte-mixture at pH ranges 2-11, 5-7 and 7-9. Peroxidase activity of the separated myoglobines of the meat samples was stained for detecting the differences in protein zones according to the keeping conditions. The myoglobin zones stained with o-dianisidin were evaluated by densitometry. The protein zones of the organic meat samples showed higher intensity than those of the control-samples.
More Related Videos
09:26Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication
Published on: September 20, 2016
05:45Capillary Electrophoresis-based Hydrogen/Deuterium Exchange for Conformational Characterization of Proteins with Top-down Mass Spectrometry
Published on: June 8, 2021
Related Concept Videos
Two-dimensional Gel Electrophoresis
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...
Background and Environment Affect Phenotype
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
Epistasis
Epistasis Analysis
SDS-PAGE
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
Position-effect Variegation