Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Maspin. A tumor suppressing serpin

R Sager1, S Sheng, P Pemberton

  • 1Division of Cancer Genetics, Dana-Farber Cancer Institute, Boston, Massachusetts 02115, USA.

Advances in Experimental Medicine and Biology
|January 1, 1997
PubMed
Summary

Maspin, a tumor suppressor in mammary cells, inhibits breast cancer invasion and metastasis. Its expression is reduced in invasive carcinomas, suggesting a key role in preventing cancer spread.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

First Measurement of Time-Dependent CP Violation in the Flavor-Changing Neutral-Current Decay B^{0}→K_{S}^{0}μ^{+}μ^{-}.

Physical review letters·2026
Same author

Measurement of the Top-Quark Production Cross Section and Charge Asymmetry at LHCb.

Physical review letters·2026
Same author

Searches for B^{0}→K^{+}π^{-}τ^{+}τ^{-} and B_{s}^{0}→K^{+}K^{-}τ^{+}τ^{-} Decays.

Physical review letters·2026
Same author

First Evidence of the B_{s}^{0}→K^{-}π^{+}γ Decay.

Physical review letters·2026
Same author

Precision Measurement of CP Violation and Branching Fractions in B^{±}→K_{S}^{0}h^{±} (h=π, K) Decays and Search for the Rare Decay B_{c}^{±}→K_{S}^{0}K^{±}.

Physical review letters·2026
Same author

First Observation of the B[over ¯]_{s}^{0}→Λ_{c}^{+}Λ[over ¯]_{c}^{-} Decay and Evidence for the B[over ¯]^{0}→Λ_{c}^{+}Λ[over ¯]_{c}^{-} Decay.

Physical review letters·2026

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Maspin is a serpin identified in mammary epithelial cells.
  • It exhibits tumor suppressor activity.
  • Maspin expression is downregulated in invasive breast carcinomas compared to normal cells.

Purpose of the Study:

  • To investigate the role of maspin in breast cancer.
  • To analyze the functional impact of maspin on tumor cell invasion and metastasis.
  • To understand the mechanism of maspin's tumor-suppressive function.

Main Methods:

  • Northern analysis to assess maspin RNA levels.
  • Immunostaining to evaluate maspin protein expression in tissues.
  • Matrigel invasion and motility assays using recombinant maspin.
  • Tumor growth and metastasis studies in nude mice with maspin-expressing transfectants.

Main Results:

  • Maspin expression is decreased in invasive breast carcinomas.
  • Recombinant maspin significantly inhibits tumor cell invasion and motility.
  • Cleavage at the reaction center abolishes maspin's inhibitory activity.
  • Tumor cells expressing maspin show reduced growth and metastasis in vivo.

Conclusions:

  • Maspin functions as a tumor suppressor in breast cancer.
  • Maspin's inhibitory effects on invasion and metastasis are dose-dependent and mechanistically linked to its reactive center.
  • Maspin's localization in the cell membrane and extracellular matrix, without classical protease inhibition, suggests a novel mechanism of action.

Related Experiment Videos