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Recognition of rheumatoid arthritis synovial antigen by CD4+,CD8- T cell clones established from rheumatoid arthritis
T Toyosaki1, Y Tsuruta, T Yoshioka
1Shionogi Research Laboratories, Shionogi & Company, Ltd., Osaka, Japan.
Objective:
To investigate the rheumatoid arthritis (RA)-specific autoantigen(s) recognized by CD4+ T cells in patients with RA.
Methods:
CD4+,CD45RO+ T cell clones were established from the joints of RA patients, and were examined for their proliferative response to synovial cells.
Results:
Eight of 146 T cell clones responded to RA synovial cells in a DR-restricted manner. These T cell clones recognized solubilized antigens extracted from RA synovial cells in the presence of DR-matched antigen-presenting cells, but did not respond to those extracted from non-RA synovial cells. The antigens had a molecular weight of 50/25 kd. Five of the 8 T cell clones used T cell receptor BV6, and the remaining clones used BV12.2.
Conclusion:
The antigens recognized by joint-infiltrating CD4+ T cells are present exclusively in RA synovial cells. The expression of these antigens by synovial cells may trigger the autoreactivity of T cells in RA joints.