Annexins II and V inhibit cell migration
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Updated: Oct 30, 2026

In vitro Cell Migration and Invasion Assays
Published on: June 1, 2014
1Department of Cellular and Molecular Physiology, University of Cincinnati College of Medicine, Ohio 45236-0576, USA.
Abstract:
Cell motility is a crucial component involved in wound healing, development, and tumor metastasis. This study investigated whether extracellular annexins, members of a calcium- and phospholipid-binding family of proteins, play a role in the migration of Lewis lung carcinoma cells. Using assays for wound closure and migration through 8-micron pores, it was found that annexins II and V significantly (> 40%) inhibited migration of these highly metastatic cells. Additionally, anti-annexin II antibodies enhanced migration of these same cells in the wound closure assay, while an irrelevant antibody (anti-calmodulin) showed no effect. These effects may be due to annexin-membrane binding and inhibition of phospholipid movement that is necessary for the formation of membrane protrusions.
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