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Related Experiment Video

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Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

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Functional association between CBP and HNF4 in trans-activation

E Yoshida¹, S Aratani, H Itou

¹Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.

Biochemical and Biophysical Research Communications

|January 22, 1998

Summary

The CREB-binding protein (CBP) coactivates transcription factors. This study reveals CBP interacts with hepatocyte nuclear factor 4 (HNF4) to enhance HNF4-mediated gene activation.

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Area of Science:

Molecular Biology
Genetics
Biochemistry

Background:

CREB-binding protein (CBP) is a crucial transcriptional coactivator.
Hepatocyte nuclear factor 4 (HNF4) is a nuclear receptor superfamily transcription factor lacking a defined ligand.

Purpose of the Study:

To investigate the interaction between CBP and HNF4.
To determine if CBP modulates HNF4 transcriptional activity.

Main Methods:

Yeast two-hybrid system for cloning HNF4 using CBP as bait.
GST-pull down assays to confirm CBP-HNF4 interaction.
Co-transfection experiments to assess transcriptional activation.

Main Results:

HNF4 was cloned using CBP in a yeast two-hybrid system, indicating an interaction.
CBP and HNF4 interact in a ligand-independent manner within specific protein domains.
CBP significantly enhances HNF4-mediated transcription.

Conclusions:

CBP and HNF4 exhibit a functional association in transcriptional regulation.
This interaction plays a role in HNF4-mediated trans-activation, independent of ligand binding.