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Related Experiment Videos

A common precursor for hematopoietic and endothelial cells

K Choi1, M Kennedy, A Kazarov

  • 1Department of Pathology, University of Maryland at Baltimore, Baltimore, MD 21201, USA. kchoi@umabnet.ab.umd.edu

Development (Cambridge, England)
|April 4, 1998
PubMed
Summary

Researchers identified the hemangioblast, a crucial precursor cell. This cell, found in embryoid bodies, gives rise to both blood and blood vessel cells, confirming a long-held hypothesis in stem cell research.

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Area of Science:

  • Developmental Biology
  • Stem Cell Biology
  • Hematopoiesis

Background:

  • Embryonic stem cells (ESCs) differentiate into various cell types within embryoid bodies (EBs).
  • A common precursor for hematopoietic and endothelial lineages has been hypothesized but not definitively identified.

Purpose of the Study:

  • To identify and characterize the common precursor cell for hematopoietic and endothelial lineages derived from ESCs.
  • To investigate the developmental potential of blast colonies formed from EB precursors.

Main Methods:

  • Culture of ESC-derived embryoid bodies.
  • Induction of blast colony formation using vascular endothelial growth factor (VEGF).
  • Analysis of cell lineage markers (CD31, flk-1, flt-1, tie-2), acetylated LDL uptake, and Weibel-Palade bodies.

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  • Cell mixing studies and kinetic analysis of precursor populations.

    • Main Results:

    • VEGF stimulation of EB precursors generated blast colonies.
    • Blast colonies yielded both hematopoietic cells and endothelial-like cells expressing specific markers.
    • Mixing studies confirmed a single cell, the blast colony-forming cell (BCFC), as the precursor for both lineages.
    • BCFC is a transient population appearing early in EB development.

    Conclusions:

    • The blast colony-forming cell represents the hemangioblast, the common embryonic precursor of hematopoietic and endothelial cells.
    • This finding provides strong evidence for the existence and role of the hemangioblast in early development.
    • Demonstrates a method for generating both lineages from a single ESC-derived precursor population.