Related Experiment Videos
A novel human luteinizing hormone receptor gene
C H Tsai-Morris1, Y Geng, E Buczko
1Section on Molecular Endocrinology, National Institute for Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.
The Journal of Clinical Endocrinology and Metabolism
|January 22, 1998
Summary
Researchers discovered a new human luteinizing hormone receptor (LHR) gene variant with distinct promoter regions. This finding suggests tissue-specific LHR gene expression may be linked to genetic diversity.
Area of Science:
- Genetics
- Molecular Biology
- Endocrinology
Background:
- The human luteinizing hormone receptor (LHR) plays a crucial role in reproductive functions.
- Previous studies have identified LHR gene sequences, but variations and their regulatory mechanisms remain incompletely understood.
Purpose of the Study:
- To isolate and characterize a novel human LHR gene from placental tissue.
- To investigate the transcriptional start sites and promoter activity of the newly identified LHR gene.
- To compare the novel LHR gene with previously identified variants and analyze its genomic context.
Main Methods:
- Genomic library screening (human placental)
- DNA sequencing and comparison
- Primer extension assays
- Reporter gene analysis
- Restriction enzyme analysis
- Gene dosing
- Chromosomal mapping
Main Results:
- A novel human LHR gene (Gene II) was isolated, differing from a previously identified gene (Gene I) in the 5' flanking region and coding sequence.
- Gene II's exon 1 coding sequence matches the human ovarian LHR cDNA sequence.
- Transcriptional start sites for Gene II and ovarian LHR mRNA are within a -176 bp TATA-less 5' flanking domain.
- Additional upstream start sites were found in testicular mRNA and choriocarcinoma cells.
- The human genome contains multiple LHR gene copies (four), unlike the rat (one copy).
- All human LHR copies map to chromosome 2p16-21.
Conclusions:
- The identified novel human LHR gene (Gene II) exhibits distinct regulatory features.
- Tissue-specific promoter utilization and transcriptional start sites contribute to LHR gene expression diversity.
- The presence of multiple LHR gene copies and their chromosomal localization suggest a complex regulatory landscape for LHR in humans.