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Glucocorticoids mediate a decrease in AVP-regulated urea transporter in diabetic rat inner medulla

J D Klein1, S R Price, J L Bailey

  • 1Department of Medicine, Emory University School of Medicine, Atlanta, Georgia 30322, USA.

The American Journal of Physiology
|January 22, 1998
PubMed
Summary
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Glucocorticoids reduce vasopressin-regulated urea transporter (VRUT) in diabetic rats. This regulation occurs independently of insulin, impacting kidney function and urea excretion.

Area of Science:

  • Nephrology
  • Endocrinology
  • Molecular Biology

Background:

  • Glucocorticoids downregulate the vasopressin-regulated urea transporter (VRUT) in adrenalectomized rats.
  • Diabetic rats exhibit increased corticosterone and urea excretion, suggesting a role for VRUT regulation.

Purpose of the Study:

  • To investigate the physiological relevance of glucocorticoid-mediated VRUT downregulation in diabetic rats.
  • To determine if glucocorticoids regulate VRUT abundance independently of insulin.

Main Methods:

  • Western analysis was used to measure VRUT protein levels in the renal inner medullary tip and base of control and streptozotocin-induced diabetic rats.
  • Experiments involved adrenalectomized rats, with and without streptozotocin and glucocorticoid replacement, to isolate the role of glucocorticoids.

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Main Results:

  • Diabetic rats showed a significant 39% reduction in VRUT protein in the renal inner medullary tip compared to controls.
  • Adrenalectomized diabetic rats treated with glucocorticoids exhibited a 32% decrease in IM tip VRUT compared to untreated adrenalectomized diabetic rats.
  • No significant difference in VRUT was observed in the inner medullary base or between adrenalectomized and adrenalectomized diabetic rats without glucocorticoid treatment.

Conclusions:

  • Glucocorticoids directly regulate the abundance of VRUT protein in the renal inner medullary tip.
  • This glucocorticoid-mediated regulation of VRUT occurs independently of insulin in the context of diabetes mellitus.