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Detection of polymorphism in BALB/c leukemia viruses with mouse antisera
Abstract:
Antisera produced in mice recognize primarily type-specific antigenic determinants on both the major core protein, p30, and the major envelope proteins, gp70 and p15(E), of the endogenous leukemia viruses (MuLV) of BALB/c mice. Three different mouse sera were investigated in detail. (i) Antisera prepared in C57BL/6 mice against the AKR leukemia K36 reacted with the gp70, p15(E), and p30 proteins of MuLV. Certain pools of the C57BL/6 anti-AKR K36 serum contained antibodies which serologically distinguished the p30 proteins of N-ecotropic, B-ecotropic, and xenotropic BALB/c MuLV. (ii) Antisera prepared in BALB/c mice against the BALB/c sarcoma 1315 contained antibodies that reacted with a type-specific antigen of the 1315 MuLV gp70 that is not found on other BALB/c MuLV. (iii) The normal sera of multiparous BALB/c mice contained antibodies that reacted with gp70 and p15(E) proteins of ecotropic MuLV. Sera from some of these mice contained antibodies that serologically distinguished the gp70 of N-ecotropic and B-ecotropic BALB/c viruses. These results emphasize the utility of mouse antisera in the serological typing of MuLV. Furthermore, the antigenic differences observed in the p30 and gp70 proteins should be of particular use in the future analysis of recombinant BALB/c MuLV.
Insights
Mouse antisera are useful for typing mouse leukemia viruses (MuLV). Specific antibodies in these sera can distinguish between different MuLV proteins, aiding in the analysis of viral strains.
Area of Science:
- Immunology
- Virology
- Molecular Biology
Background:
- Endogenous mouse leukemia viruses (MuLV) possess type-specific antigenic determinants on core (p30) and envelope (gp70, p15(E)) proteins.
- Previous studies utilized mouse antisera for MuLV characterization, but detailed analysis of specific antibody reactivity is ongoing.
Purpose of the Study:
- To investigate the utility of mouse antisera for serological typing of BALB/c mouse leukemia viruses (MuLV).
- To identify specific antigenic differences in MuLV proteins (p30, gp70, p15(E)) using various mouse antisera.
Main Methods:
- Production and detailed analysis of three distinct mouse antisera: C57BL/6 anti-AKR K36, BALB/c anti-BALB/c sarcoma 1315, and normal sera from multiparous BALB/c mice.
- Serological assays to detect antibody binding to MuLV proteins (p30, gp70, p15(E)) and differentiate between N-ecotropic, B-ecotropic, and xenotropic MuLV strains.
Main Results:
- Mouse antisera recognized type-specific determinants on MuLV p30, gp70, and p15(E) proteins.
- Specific antibody pools distinguished N-ecotropic, B-ecotropic, and xenotropic BALB/c MuLV p30 proteins.
- Antisera identified a type-specific antigen on BALB/c sarcoma 1315 MuLV gp70.
- Normal mouse sera detected antibodies against ecotropic MuLV gp70 and p15(E), with some differentiating N- and B-ecotropic gp70.
Conclusions:
- Mouse antisera are valuable tools for the serological classification of MuLV.
- Observed antigenic differences in p30 and gp70 proteins offer potential for future analysis of recombinant MuLV strains.