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Comparative analysis of equine lymphocyte subsets in whole blood and gradient-purified samples
M K Akens1, E Holznagel, M Franchini
1Department of Veterinary Internal Medicine, University of Zurich, Switzerland. makens@vetklinik.unizh.ch
Veterinary Immunology and Immunopathology
|January 22, 1998
Abstract:
In the present study, two methods of lymphocyte preparation, whole blood lysis and Ficoll-Paque separation, prior to FACS analysis were compared. The comparison was done with single and dual-colour staining techniques. Monoclonal antibodies (mAb) against eCD4, eCD5, eCD8 and eMHC class II were used. There was no significant difference in the results obtained by these two methods.