Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Cell-biological changes on the disordered tissues]

T Tsuji1

  • 1Department of Legal Medicine, Wakayama Medical College, Japan.

Nihon Hoigaku Zasshi = the Japanese Journal of Legal Medicine
|January 22, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quantitative HCV RNA and effect of interferon therapy in chronic hepatitis C.

Digestive diseases and sciences·1992
Same author

Measurement of proliferating cell nuclear antigen (PCNA) and its clinical application in oral cancers.

International journal of oral and maxillofacial surgery·1992
Same author

Suprachiasmal carotid-ophthalmic artery aneurysm--report of two cases.

Neurologia medico-chirurgica·1992
Same author

Histological pancreatitis in end-stage renal disease.

International journal of pancreatology : official journal of the International Association of Pancreatology·1992
Same author

Application of monoclonal antibodies to blood grouping of mixed body fluids.

Nihon hoigaku zasshi = The Japanese journal of legal medicine·1992
Same author

Amino acids and peptides. XVI. Synthesis of N-terminal tetrapeptide analogs of fibrin alpha-chain and their inhibitory effects on fibrinogen/thrombin clotting.

Chemical & pharmaceutical bulletin·1992
Same journal

[Abstracts of the 99th Congress of the Japanese Society of Legal Medicine. June 10-12, 2015, Kouchi, Japan].

Nihon hoigaku zasshi = The Japanese journal of legal medicine·2015
Same journal

[Abstract of the branch meetings of the Japanese Society of Legal Medicine. 2013, Japan].

Nihon hoigaku zasshi = The Japanese journal of legal medicine·2014
Same journal

[Abstracts of the 97th Congress of the Japanese Society of Legal Medicine. June 26-28, 2013, Sapporo, Japan].

Nihon hoigaku zasshi = The Japanese journal of legal medicine·2013
Same journal

[Abstract of the branch meetings of the Japanese Society of Legal Medicine. 2012, Japan].

Nihon hoigaku zasshi = The Japanese journal of legal medicine·2013
Same journal

[Abstract of the branch meetings of the Japanese Society of Legal Medicine. 2010, Japan].

Nihon hoigaku zasshi = The Japanese journal of legal medicine·2012
Same journal

[Resident training program of coroner's office and trauma quality assurance in Los Angeles County, California, USA].

Nihon hoigaku zasshi = The Japanese journal of legal medicine·2011
See all related articles

This study reveals heat-induced liver injury in rats involves apoptosis and necrosis, with apoptosis occurring in a bimodal pattern. Neutrophil infiltration and TNF-alpha appear crucial in later stages of cell death.

Area of Science:

  • Cell Biology
  • Histopathology
  • Molecular Biology

Background:

  • Investigated mechanisms of cell death (necrosis and apoptosis) following thermal injury in rat liver.
  • Developed a novel heat device with PID control for precise temperature regulation during experiments.

Discussion:

  • Apoptosis exhibited a bimodal pattern at 2 and 10 hours post-heating across all temperatures (37°C, 50°C, 65°C).
  • Temperature-dependent differences in apoptosis onset and progression were observed, with higher temperatures causing distinct patterns.
  • Heat shock protein (HSP) expression coincided with the early apoptosis peak, suggesting a direct thermal response.

Key Insights:

  • The first apoptosis peak appears to be a direct response to temperature, while the second peak correlates with neutrophil infiltration and TNF-alpha release.

Related Experiment Videos

  • Necrosis occurred alongside apoptosis, with apoptotic cells being phagocytosed and only necrotic tissue remaining after 24 hours.
  • TNF-alpha, likely derived from neutrophils, is implicated in the generation of both necrosis and apoptosis.

    • Outlook:

    • Further research could explore therapeutic interventions targeting neutrophil-mediated pathways to mitigate thermal injury.
    • Investigating the precise molecular signaling cascades initiated by TNF-alpha in this context is warranted.
    • Comparative studies on different tissue types or injury models could elucidate broader principles of thermal cell death.