Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Isolation of basic protein-acidic lipid complex from myelin

T Kunishita, K Uemura, A Okano

    The Japanese Journal of Experimental Medicine
    |December 1, 1979
    PubMed
    Summary

    Canine myelin was extracted to isolate a basic protein-acidic lipid complex, revealing its composition and encephalitogenic activity. This complex may play a role in myelin's molecular organization.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Impact of an ultrasound-guided radiofrequency ablation training program on the outcomes in patients with hepatocellular carcinoma.

    Diagnostic and interventional imaging·2019
    Same author

    Gastrointestinal: Improvement of refractory oral ulcer of Behçet's disease after eradication of Helicobacter pylori.

    Journal of gastroenterology and hepatology·2018
    Same author

    The effects of thalidomide on chemotactic migration of multiple myeloma cell lines.

    International journal of laboratory hematology·2008
    Same author

    Expression and role of MHC class I-related chain in myeloma cells.

    Cytotherapy·2006
    Same author

    Rituximab is effective for steroid-refractory sclerodermatous chronic graft-versus-host disease.

    Leukemia·2005
    Same author

    High serum bone-specific alkaline phosphatase level after bortezomib-combined therapy in refractory multiple myeloma: possible role of bortezomib on osteoblast differentiation.

    Leukemia·2005

    Area of Science:

    • Biochemistry
    • Neuroscience
    • Molecular Biology

    Background:

    • Myelin, the insulating sheath around nerve fibers, is crucial for rapid signal transmission.
    • Understanding myelin composition is key to understanding neurological function and disease.
    • Proteolipid protein and basic protein are major myelin proteins.

    Purpose of the Study:

    • To characterize the lipid and protein components of canine brain myelin.
    • To investigate the interaction between basic protein and acidic lipids in myelin.
    • To assess the encephalitogenic activity of a basic protein-acidic lipid complex.

    Main Methods:

    • Lyophilized canine myelin extraction using n-propanol-n-hexane and chloroform-methanol solvent mixtures.
    • UV-spectroscopy, Thin-Layer Chromatography (TLC), and SDS-gel electrophoresis for component analysis.
    • Amino acid composition analysis and fatty acid profiling of isolated lipids.

    Main Results:

    • A chloroform-methanol extract of myelin residue was rich in sulfatide (63.6%) and phosphatidylserine (26.2%).
    • The protein component was primarily basic protein, with amino acid composition similar to porcine basic protein.
    • A basic protein-acidic lipid complex (2.56:1 protein to lipid ratio) was identified, exhibiting encephalitogenic activity.

    Conclusions:

    • Canine myelin contains a significant basic protein-acidic lipid complex.
    • This complex's solubilization suggests interaction between basic protein and acidic lipids, potentially influencing myelin structure.
    • The identified complex possesses encephalitogenic properties, distinct from basic protein alone.

    Related Experiment Videos