31P NMR investigation of vanadium-treated chick muscle
1Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA.
Insights
Dietary vanadium in chicks significantly lowered breast muscle pH, impacting growth. Phosphorus NMR revealed muscle differences but only pH was notably affected by vanadium.
Area of Science:
- Biochemistry
- Animal Physiology
- Nuclear Magnetic Resonance (NMR) Spectroscopy
Background:
- Muscle differentiation involves changes in pH, metabolites, and energy.
- Vanadium is a trace element with potential biological effects.
Purpose of the Study:
- To investigate the in vivo phosphorus NMR profiles of normal and vanadium-treated chick muscle.
- To assess the impact of dietary vanadium on muscle differentiation and bioenergetic parameters.
Main Methods:
- In vivo phosphorus Nuclear Magnetic Resonance (NMR) spectroscopy was performed on chick breast and thigh muscles.
- Measurements included pH, lipid-related metabolites, and bioenergetic parameters.
Main Results:
- Phosphorus NMR successfully differentiated breast and thigh muscles based on pH, metabolites, and energy.
- Vanadium-treated chicks exhibited growth retardation.
- Dietary vanadium significantly decreased the pH of breast muscle, making it more acidic.
Conclusions:
- In vivo phosphorus NMR is a valuable tool for studying muscle differentiation and metabolic changes.
- Dietary vanadium primarily affects breast muscle acidity, despite causing overall growth retardation.
Abstract:
The phosphorus NMR profile of normal and vanadium-treated chick muscle was obtained in vivo. The data show that the differentiation of breast and thigh muscles in terms of pH, lipid related metabolites, and bioenergetic parameters can be readily followed. Although the vanadium-treated chicks showed substantial retardation of growth, the only NMR parameter that was significantly affected by dietary vanadium was the pH of breast muscle, which was substantially more acidic in the vanadium-treated animals.
