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Factor V

J Rosing1, G Tans

  • 1Department of Biochemistry, Maastricht University, The Netherlands.

The International Journal of Biochemistry & Cell Biology
|January 23, 1998
PubMed
Summary
This summary is machine-generated.

Factor V (FV) is crucial for blood clotting, acting as a cofactor that significantly speeds up thrombin generation. Genetic resistance to activated protein C (APC) inactivation of factor FVa causes hereditary thrombophilia.

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Area of Science:

  • Biochemistry
  • Hematology
  • Molecular Biology

Background:

  • Factor V is a glycoprotein essential for regulating blood coagulation.
  • Activated factor V (factor Va) serves as a critical protein cofactor in the prothrombin-activating complex.

Purpose of the Study:

  • To elucidate the role of factor Va in accelerating prothrombin activation.
  • To understand the regulatory mechanism of factor Va activity by activated protein C (APC).
  • To investigate the genetic basis of hereditary thrombophilia related to APC resistance.

Main Methods:

  • Limited proteolysis converts factor V to factor Va.
  • Factor Va functions within the prothrombin-activating complex involving factor Xa, Ca2+ ions, and a membrane surface.
  • The activity of factor Va is down-regulated by APC.

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Main Results:

  • Factor Va enhances factor Xa-catalyzed prothrombin to thrombin conversion by over 10,000-fold.
  • APC inactivates factor Va, thereby regulating coagulation.
  • Genetic defects causing APC resistance in factor Va lead to hereditary thrombophilia.

Conclusions:

  • Factor Va is a key accelerator of thrombin generation.
  • APC-mediated regulation of factor Va is vital for preventing thrombophilia.
  • Understanding APC resistance is crucial for diagnosing and managing hereditary thrombophilia.