Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

(+)-Catechin prevents human plasma oxidation

S B Lotito1, C G Fraga

  • 1Fisicoquímica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.

Free Radical Biology & Medicine
|January 23, 1998
PubMed
Summary

(+)-Catechin (CTCH) effectively protects human plasma against oxidation by free radicals. This antioxidant delays lipid oxidation and preserves endogenous antioxidants like alpha-tocopherol and beta-carotene.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Flavonoids and the gastrointestinal tract: Local and systemic effects.

Molecular aspects of medicine·2018
Same author

Angiotensin II blockade improves mitochondrial function in spontaneously hypertensive rats.

Cellular and molecular biology (Noisy-le-Grand, France)·2005
Same author

The interaction of flavonoids with membranes: potential determinant of flavonoid antioxidant effects.

Free radical research·2005
Same author

Ascorbate protects (+)-catechin from oxidation both in a pure chemical system and human plasma.

Biological research·2005
Same author

Oxidative stress in aging and disease.

Molecular aspects of medicine·2004
Same author

Comparison between the antioxidant status of terrestrial and diving mammals.

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology·2002

Area of Science:

  • Biochemistry
  • Oxidative Stress Research
  • Nutritional Science

Background:

  • Free radicals cause damage to biological systems.
  • Human plasma is susceptible to oxidation, impacting health.
  • (+)-Catechin (CTCH) is known for its antioxidant properties.

Purpose of the Study:

  • To investigate the protective role of CTCH against oxidation in human plasma.
  • To determine CTCH's effect on lipid oxidation and endogenous antioxidants.

Main Methods:

  • Human plasma samples were exposed to radical initiators (AAPH or AMVN).
  • Lipid oxidation was measured using TBARS assay.
  • Alpha-tocopherol, beta-carotene, and CTCH levels were quantified via HPLC.

Main Results:

  • AAPH and AMVN induced significant lipid oxidation and depleted plasma alpha-tocopherol and beta-carotene.
  • CTCH addition dose-dependently prevented TBARS formation and protected alpha-tocopherol and beta-carotene.
  • CTCH altered the lag time and consumption rate of endogenous antioxidants.

Conclusions:

  • CTCH is a potent antioxidant in human plasma.
  • It inhibits lipid oxidation and delays the depletion of lipid-soluble antioxidants.
  • CTCH's efficacy depends on its concentration and the nature of the radical initiator.

Related Experiment Videos