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Effects of sequential reagent addition on technetium-99m red blood cell labeling efficiency using the UltraTag kit
J Massler1, C Mento, S Shodavaram
1Department of Radiology, College of Physicians and Surgeons, Columbia University, New York, New York, USA.
Objective:
The purpose of this study was to investigate how the reagent-adding sequence affects 99mTc red blood cell (RBC) labeling efficiency using the UltraTag RBC kit.
Methods:
The package insert for preparation and quality control was followed during the experiment. The reagent-adding sequences were then altered during preparation.
Results:
If acid-citrate dextrose is added before the NaOCl, the labeling efficiency decreases dramatically. However, the order of addition of Na 99mTcO4 and acid-citrate dextrose did not affect the labeling efficiency.
Conclusion:
Addition of the NaOCl solution before the acid-citrate dextrose solution is imperative for proper labeling efficiency.