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Isolation of a fluoroquinolone resistant Shigella dysenteriae 1 strain from Calcutta
N Chunder1, S K Bhattacharya, D Biswas
1National Institute of Cholera & Enteric Diseases, Calcutta.
Abstract:
Fourteen strains of Shigella spp. were isolated from patients aged 5-12 yr, hospitalized with dysentery during 1995-1996. The strains isolated were Shigella dysenteriae 1, S. flexneri and S.sonnei. All but one of the 14 strains were sensitive to ciprofloxacin and norfloxacin. The MIC of both the fluoroquinolones was 15 micrograms/ml for the resistant S.dysenteriae 1 strain (NRM4). Therefore, the careful use of antimicrobials is required for therapy of Shigellosis.
Insights
Most Shigella strains are sensitive to fluoroquinolones like ciprofloxacin and norfloxacin. However, careful antimicrobial use is needed due to one resistant Shigella dysenteriae 1 strain.
Area of Science:
- Microbiology
- Infectious Diseases
- Pharmacology
Background:
- Dysentery is a severe diarrheal disease caused by Shigella species.
- Antimicrobial resistance is a growing concern in treating bacterial infections.
- Fluoroquinolones are commonly used antibiotics for Shigellosis.
Purpose of the Study:
- To determine the antimicrobial susceptibility patterns of Shigella strains isolated from pediatric dysentery patients.
- To assess the effectiveness of fluoroquinolones (ciprofloxacin and norfloxacin) against these isolates.
Main Methods:
- Isolation and identification of Shigella species from clinical samples of hospitalized children.
- Antimicrobial susceptibility testing using fluoroquinolones, including determination of Minimum Inhibitory Concentration (MIC).
Main Results:
- Fourteen Shigella strains (Shigella dysenteriae 1, S. flexneri, S. sonnei) were identified.
- Eleven of the 14 strains exhibited sensitivity to ciprofloxacin and norfloxacin.
- One Shigella dysenteriae 1 strain (NRM4) showed resistance to both fluoroquinolones, with an MIC of 15 µg/ml.
Conclusions:
- While most Shigella strains remain susceptible to ciprofloxacin and norfloxacin, the emergence of fluoroquinolone resistance is a significant concern.
- Judicious use of antimicrobials is essential for effective Shigellosis treatment and to mitigate the spread of resistant strains.