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The human histone gene cluster at the D6S105 locus
1Institut für Biochemie und Molekulare Zellbiologie, Universität Göttingen, Germany.
Human Genetics
|January 24, 1998
Summary
Researchers mapped human histone genes within a cluster at D6S105, identifying 16 genes and 2 pseudogenes. This analysis completes the inventory of human histone genes, enabling a comprehensive overview of their organization.
Area of Science:
- Genomics
- Molecular Biology
- Human Genetics
Background:
- Histone genes are crucial for DNA packaging and regulation.
- Understanding the organization of histone gene clusters is essential for comprehending genome structure and function.
Purpose of the Study:
- To determine the sequences and organization of histone genes within the cluster at chromosomal marker D6S105.
- To complete the set of identified human histone genes and enable a comprehensive outline of their genomic organization.
Main Methods:
- Analysis of Centre d'Etude du Polymorphisme Humain yeast artificial chromosome (YAC) 964f1.
- Subcloning of the YAC insert into cosmids and establishment of a contig.
- Identification and characterization of histone genes and pseudogenes within the contig.
Main Results:
- A histone gene cluster of approximately 80 kb was characterized at D6S105.
- The cluster contains 16 histone genes (1 H1, 5 H2A, 4 H2B, 3 H3, 3 H4) and 2 pseudogenes (1 H2B, 1 H4).
- The arrangement of histone genes within the cluster is non-ordered, with the D6S105 marker at its telomeric end.
Conclusions:
- The identified histone genes and pseudogenes, along with previously known loci, represent the complete set of human histone genes.
- This comprehensive data allows for the outlining of the general organization of the human histone gene complement.
- The findings facilitate further studies on histone gene regulation and evolution.