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Decrease in class pi glutathione transferase mRNA levels by ultraviolet irradiation of cultured rat keratinocytes
1Second Department of Biochemistry, Hirosaki University School of Medicine.
Japanese Journal of Cancer Research : Gann
|January 24, 1998
Summary
Ultraviolet (UV) B irradiation significantly decreases pi class glutathione transferase (GST-P) gene expression in rat keratinocytes. This UV-induced repression of GST-P is not mediated by changes in c-Jun or c-Fos mRNA levels.
Area of Science:
- Molecular Biology
- Dermatology
- Toxicology
Background:
- Glutathione transferase pi (GST-P) is a key enzyme in cellular detoxification.
- UVB irradiation is a known environmental stressor affecting skin cells.
- Understanding gene regulation in response to UV damage is crucial for skin health.
Purpose of the Study:
- To investigate the impact of UVB irradiation on GST-P gene expression in cultured rat keratinocytes.
- To explore the underlying molecular mechanisms, including the role of trans-acting factors.
Main Methods:
- Cultured rat keratinocytes were exposed to UVB irradiation.
- Gene expression levels of GST-P, c-Jun, and c-Fos were analyzed using immunoblotting and Northern blot.
- Reporter gene assays (CAT) were performed to assess the role of regulatory regions.
Main Results:
- UVB irradiation significantly reduced GST-P mRNA levels to 10-25% of initial values 24 hours post-exposure.
- Hydrogen peroxide treatment, conversely, enhanced GST-P mRNA expression.
- Changes in c-Jun and c-Fos mRNA levels did not correlate with the repression of GST-P mRNA, and reporter gene activity was not suppressed by UV.
Conclusions:
- UVB irradiation represses GST-P gene expression in rat keratinocytes.
- The observed repression is not directly mediated by alterations in c-Jun, c-Fos, or the characterized regulatory regions of the GST-P gene.
- The precise mechanism of UVB-induced GST-P downregulation remains to be elucidated.