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Related Experiment Videos

The membrane transport of ascorbic acid

G V Mann, P Newton

    Annals of the New York Academy of Sciences
    |September 30, 1975
    PubMed
    Summary
    This summary is machine-generated.

    High blood sugar levels, or hyperglycemia, impair vitamin C transport into human red blood cells. This reduced transport of dehydroascorbate (DHA) suggests lower intracellular vitamin C availability in conditions of hyperglycemia.

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    Area of Science:

    • Biochemistry and Cellular Physiology
    • Human Metabolism and Nutrition

    Background:

    • Understanding the transport mechanisms of vital nutrients like vitamin C is crucial for human health.
    • Dehydroascorbate (DHA) is the oxidized form of vitamin C and its transport into cells is a key step in maintaining intracellular vitamin C levels.

    Purpose of the Study:

    • To investigate the kinetics of dehydroascorbate (DHA) transport into human red blood cells.
    • To determine the influence of various sugars and copper ions on DHA transport.
    • To evaluate the implications of these findings for vitamin C availability under hyperglycemic conditions.

    Main Methods:

    • Utilized a system to measure the rate of DHA transport into human red blood cells.
    • Assessed Michaelis-Menten kinetics and substrate inhibition patterns for DHA transport.

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  • Examined the effects of different sugars (D-glucose, D-mannose, etc.) and copper ions on DHA uptake.

    • Main Results:

    • DHA transport into red blood cells follows Michaelis-Menten kinetics with substrate inhibition above 150 muM DHA.
    • Sugars significantly impair DHA transport, with D-glucose having the most pronounced effect at physiological concentrations.
    • Copper ions accelerate DHA transport, enabling movement against a concentration gradient.

    Conclusions:

    • Hyperglycemia likely impairs the intracellular availability of vitamin C by inhibiting DHA transport.
    • The findings support the hypothesis that elevated blood glucose levels negatively impact cellular vitamin C status.
    • Understanding these transport dynamics is vital for metabolic health and nutritional interventions.